Abstract
Appropriate strategies are required for alleviation of hypothermia and hyperglycemia over general anesthesia in mammals. The present study aimed to explore changes in thermos-tolerance response, blood and plasma parameters upon supplementation of Moringa oleifera (M. oleifera 25.0% vs. 50.0 %) of non-anesthetized and anesthetized mice using diazepam and xylazine (DX; 13.3 mg/kg BW diazepam and 26.6 mg/kg BW xylazine). Thirty six adult albino male mice of 32.72 +/- 0.32 g body weight and three months old age were classified into three groups; control group (G1) fed basal control diet versus two M. oleifera groups fed basal control diet containing M. oleifera (G2; 25.0% and G3; 50.0%) for four weeks. Thermos-tolerance (rectal temperature, pulse rates, partial pressure of oxygen) and glucose values were determined before (0 min) and 20 min, 40 min, 1h, 2h, 3h and 4h after DX injection of control and M. oleifera groups. In addition, blood samples were collected two days before and 4h after DX injection and were subjected for analysis through hematology and plasma biochemistry analyzers. The hematological parameters include red and white blood cells, hematocrit and hemoglobin values. The plasma biochemistry parameters include total proteins, albumin, blood urea nitrogen and phosphorus values. The results indicated that M. oleifera caused positive changes in thermo-tolerance (rectal temperature, pulse rate, and SPO2), hematological (RBCs, HGB, HCT and WBCs) and plasma biochemistry (total protein, albumin, glucose and blood urea nitrogen) parameters compared to control diet either before or after DX injection. It could be concluded that M. oleifera (25.0 & 50.0 %) might alleviate the transient negative side effects of hypothermia and hyperglycemia due to general anesthesia using DX in mice through modulating thermo-tolerance responses, blood indices and plasma metabolites.