Abstract
Simple Summary The reproducibility of handcrafted radiomic features (HRFs) has been reported to be affected by variations in imaging acquisition and reconstruction parameters. However, to date, these effects have not been understood or quantified. In this study, we analyzed a significantly large number of scenarios in an effort to quantify the effects of variations on the reproducibility of HRFs. In addition, we assessed the performance of ComBat harmonization in each of the 31,375 investigated scenarios. We developed a novel score that can be considered the first attempt to objectively assess the number of reproducible HRFs in different scenario. Following further validation, the score could be used to decide on the inclusion of data acquired differently, as well as the assessment of the generalizability of developed radiomic signatures. The reproducibility of handcrafted radiomic features (HRFs) has been reported to be affected by variations in imaging parameters, which significantly affect the generalizability of developed signatures and translation to clinical practice. However, the collective effect of the variations in imaging parameters on the reproducibility of HRFs remains unclear, with no objective measure to assess it in the absence of reproducibility analysis. We assessed these effects of variations in a large number of scenarios and developed the first quantitative score to assess the reproducibility of CT-based HRFs without the need for phantom or reproducibility studies. We further assessed the potential of image resampling and ComBat harmonization for removing these effects. Our findings suggest a need for radiomics-specific harmonization methods. Our developed score should be considered as a first attempt to introduce comprehensive metrics to quantify the reproducibility of CT-based handcrafted radiomic features. More research is warranted to demonstrate its validity in clinical contexts and to further improve it, possibly by the incorporation of more realistic situations, which better reflect real patients' situations.