Abstract
A segregating F4 population from the cross between drought sensitive (Yecora Rojo) and drought tolerant (Pavon 76) genotypes was made to identify molecular markers linked to a wheat (
Triticum aestivum
L.) abscisic acid (ABA) content at two water regimes. The parents and 150 F
4
lines were evaluated phenotypically for drought tolerance using two irrigation treatments [0.25 and 0.75 m
3
(H
2
O) m
−2
(soil)]. Forty different target region amplification polymorphism (TRAP) primer combinations, 98 different sequence-related amplified polymorphism (SRAP) primer combinations, and 400 simple sequence repeat (SSR) primers were tested for polymorphism among the parental genotypes and the F
4
lines. Seven loci in the F
4
lines treated with the drought stress were identified. Single quantitative trait loci (QTLs) were located on chromosomes 1B, 2A, 3A, 5D, and 7B and each of them explained from 15 to 31 % of phenotypic variance with a LOD value of 7.2 to 15.7. Five QTLs were located on chromosome 4A and six QTLs on chromosome 5A. In control (well-watered) F
4
lines, two QTLs were mapped on chromosome 3B and one QTL on each chromosome 5B and 5D. Statistically the most significant groups of QTLs for the ABA content were identified in the regions of chromosomes 3B, 4A, and 5A mostly near to Barc164, Wmc96, and Trap9 markers. Therefore, these markers linked to QTLs for the drought-induced ABA content can be further used in breeding for drought tolerance in wheat.