Abstract
Cell Division Cycle Associated 5 (
) plays a role in the phosphoinositide 3-kinase (PI3K)/AKT/mTOR signalling pathway involving cell division, cancer cell migration and apoptosis. This study aims to assess the prognostic and biological value of
in breast cancer (BC).
The biological and prognostic value of
were evaluated at mRNA (
= 5109) and protein levels (
= 614) utilizing multiple well-characterized early stage BC cohorts. The effects of
knockdown (KD) on multiple oncogenic assays were assessed in vitro using a panel of BC cell lines.
this study examined cohorts showed that high
expression was correlated with features characteristic of aggressive behavior and poor prognosis, including the presence of high grade, large tumor size, lymphovascular invasion (LVI), hormone receptor negativity and HER2 positivity. High
expression, at both mRNA and protein levels, was associated with shorter BC-specific survival independent of other variables (
= 0.034, Hazard ratio (HR) = 1.6, 95% CI; 1.1-2.3). In line with the clinical data, in vitro models indicated that
depletion results in a marked decrease in BC cell invasion and migration abilities and a significant accumulation of the BC cells in the G2/M-phase.
These results provide evidence that
plays an important role in BC development and metastasis and could be used as a potential biomarker to predict disease progression in BC.