Abstract
► A membrane filtration procedure for determination of Sudan orange G is presented. ► Analytical conditions like pH, flow rates, type of filter were investigated. ► The validation of the procedure was performed by the method of standard addition. ► The procedure was applied to chili powder and sauce, tomato sauce and water.
A simple membrane filtration procedure for separation–enrichment of Sudan orange G is presented. The method is based on the adsorption of Sudan orange G on a cellulose acetate filter and its elution from the membrane with 10mL of ethanol. Sudan orange G in the eluent was determined by UV–visible spectrophotometry at 388nm. The effect of analytical conditions, including pH, flow rates and eluent, sample volume, type of membrane for quantitative preconcentration and separation of Sudan orange G were examined. The influences of matrix components on Sudan orange G recoveries were studied. The preconcentration factor was 125. The detection limit was 4.9μgL−1. The relative standard deviation was 4.3%. The presented procedure was applied to chili powder, chili sauce, tomato sauce, powdered beverage and water samples.