Abstract
Arboviral diseases and microbial pathogens resistant to commercially available drugs are on the rise. Herein, a facile microbial-based approach was developed to synthesize selenium nanowires (Se NWs) using microbial exopolymer (MEP) extracted from the Bacillus licheniformis (probiotic bacteria). MEP-Se NWs were characterized using UV–Visible, XRD, FTIR, HR-TEM, FE-SEM and EDX. An UV–Visible peak was detected at 330 nm while XRD spectrum data pointed out the crystalline nature of MEP-Se NWs. FTIR spectrum revealed functional groups with strong absorption peaks in the range 3898.52–477.97 cm−1. FE-SEM and HR-TEM revealed that the obtained structures were nanowires of 10–30 nm diameter. Se presence was confirmed by EDX analysis. MEP-Se NWs at 100 μg/ml highly suppressed the growth of both Gram (−) and Gram (+) bacteria. Further, microscopic analysis evidenced that 75 μg/ml MEP-Se NWs suppressed biofilm formation. Hemolytic assays showed that MEP-Se NWs were moderately cytotoxic. In addition, LC50 values lower than 10 μg/ml were estimated testing MEP-Se NWs on both Aedes aegypti and Culex quinquefasciatus 3rd instar larvae. Morphological and histological techniques were used to elucidate on the damages triggered in mosquito tissues, with special reference to midgut, post-exposure to MEP-Se NWs. Therefore, based on our findings, MEP-Se NWs can be considered for entomological and biomedical applications, with special reference to the management of biofilm forming microbial pathogens and arbovirus mosquito vectors.
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•Se nanowires (Se NWs) were synthesized using microbial exopolymers (MEP) from Bacillus licheniformis Dahb1.•MEP-Se NWs were characterized by UV–Vis, XRD, FTIR, FE-SEM, HR-TEM and EDX.•MEP-Se NWs revealed great antioxidant and antibiofilm activity.•Low hemolytic activity of MEP-Se NWs pointed out their biocompatible nature.•MEP-Se NWs were highly toxic to Aedes aegypti and Culex quinquefasicatus larvae.