Abstract
Organometallic macromolecules such as ferrocenyl bis-pyrazoline (2, 3) and bis-pyrimidine (4, 5) derivatives were synthesized by reacting ferrocenyl bis-chalcone 1 with thiosemicarbazide/phenylhydrazine/guanidine hydrochloride/thiourea, respectively, under microwave irradiation. Ferrocenyl bis-chalcone 1 was synthesized by reacting acetyl ferrocene with terephthalaldehyde. Synthesized compounds were characterized by using IR, H-1 NMR, C-13 NMR, EI-MS, and elemental analysis. In vitro antibacterial activity against two Gram-negative and two Gram-positive bacteria was determined by the disc diffusion assay. Moreover, minimum inhibition concentrations were also measured with reference to chloramphenicol. Thioamide functionally containing ferrocenyl bis-pyrazoline derivative 2 shows the best antibacterial activity on Escherichia coli and Salmonella typhimurium, among all tested compounds including the reference drug chloramphenicol. The structure-activity relationship is also developed by using computational calculations with density functional theory (DFT)/B3LYP method.