Abstract
Objectives: In parenteral nutrition-dependent infants and children, intestinal failure (IF)-associated liver disease (IFALD) remains an important problem. A comparative study was undertaken of parenteral mixed lipid (ML), omega-3 predominant fish oil (FO), and omega-6 predominant soybean oil (SO) emulsions in regards to hepatic phytosterol, neutral lipid, fatty acid (FA) content, and the relationship to cholestasis in piglets.
Methods: Neonatal piglets received parenteral nutrition, varying in lipid dose (5 or 10 g . kg(-1) . day(-1)) and formulation: SO5 (n = 5), SO10 (n = 5), FO5 (n = 5), and ML10 (n = 5). On day 14, liver chemistry, bile flow, histology and neutral lipid staining were assessed. Hepatic triglyceride FA content was determined using thin layer and gas chromatography, and phytosterol content was assessed using gas chromatography-mass spectrometry.
Results: SO groups had higher prevalence of biochemical cholestasis (P<0.04) and lower bile flow (P<0.0001). Hepatic campesterol, stigmasterol, and beta-sitosterol were highest in SO10 (P<0.0001). Hepatic FA (P<0.03) and omega-6/omega-3 FA ratio( P<0.0001) were higher in the SO groups. Neutral lipid accumulation (P = 0.3) and liver histology (P = 0.16) were not different between groups. Univariate predictors of bile flow were: campesterol (r = -0.77, P = 0.001), beta-sitosterol (r = -0.74, P = 0.002), stigmasterol (r = -0.74, P = 0.002), omega-6 FA (r = -0.72, P = 0.002), and omega-3 FA (r = 0.59, P = 0.02). Only campesterol independently predicted bile flow.
Conclusions: ML and FO lipid emulsions reduce cholestasis in association with lowered hepatic phytosterol and lipid content. Lower hepatic phytosterol and omega-6 FA content, and higher omega-3 FA content are hepatoprotective. Multivariate analysis suggests reduced phytosterol accumulation may best explain the hepatoprotective effect of fish oil-containing lipids.