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Mixed SAMs and MALDI-ToF MS: preparation of N-glycosylamine derivative and thioctic acid methyl ester bearing 1,2-dithiolane groups and detection of enzymatic reaction on Au
Journal article   Peer reviewed

Mixed SAMs and MALDI-ToF MS: preparation of N-glycosylamine derivative and thioctic acid methyl ester bearing 1,2-dithiolane groups and detection of enzymatic reaction on Au

Hani Mutlak A Hassan and Beatrice A Maltman
Bioorganic chemistry, Vol.40(1), pp.6-9
02/2012
DOI: https://doi.org/10.1016/j.bioorg.2011.10.004
PMID: 22130071

Abstract

Acetylglucosamine - analogs & derivatives Acetylglucosamine - chemical synthesis Acetylglucosamine - chemistry Amines - chemistry Enzymes - metabolism Gold - chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Surface Properties Thioctic Acid - analogs & derivatives Thioctic Acid - chemical synthesis Thioctic Acid - chemistry
Herein, we report an enzymatic galactosylation reaction of β-glucopyranosylamide 4 and thioctic acid methyl ester 5 bearing 1,2-dithiolane groups to form a new system of mixed self-assembled monolayers (SAMs) on gold. Characterization of the enzymatic activity was conveniently achieved by mass spectrometry.

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