Abstract
•Polysaccharide was extracted from Gossypium arboreum L. seed.•Central composite rotatable response surface design was employed in this study.•Developed model was confirmed as a good fitting model via ANOVA.•Optimal conditions were determined by numerical optimization method.•FTIR was used to analyze the extracted polysaccharide.
The present study investigates the effect of independent variables such as extraction temperature (35–55°C), time (1–5h) and solid–liquid ratio (1:5–1:25g/ml) over the extraction yield of polysaccharide from Gossypium arboreum L. seeds was investigated and optimized. Aqueous extraction method was opted for the extraction of polysaccharide. Central composite response surface design was utilized for developing the experimental design. A second order polynomial mathematical model was developed from the obtained results. From the results, Significance of process variables over the extraction process can be clearly depicted. At the extraction temperature of 45°C, extraction time of 3h and solid–liquid ratio of 1:15g/ml maximum yield of polysaccharide (8.67%) from Gossypium arboreum L. seed was obtained. Characteristics of the extracted polysaccharide are analyzed through physico-chemical property analysis and Fourier Transform Infrared Spectroscopy (FTIR).