Abstract
Candida albicans is a widespread opportunistic pathogen in immuno-compromised patients. ATPase activity and protein concentration using spectrophotometric method and antifungal activity by Microtitre / ELISA technique in terms of Minimum Inhibitory Concentration (MIC) was studied to understand the pattern of Candida albicans. Minimum Inhibitory Concentration for Clotrimazole and Nystatin showed 6.25 mu g/ml and 8.0 mu g/ml "respectively". At optimum pH (7.0) and temperature (28 degrees C), 100% activity of ATPase was recorded. pH showed inverse relationship while temperature showed no remarkable variation of activity. ATPase was found to be 5.0% reduced by Clotrimazole, whereas Nystatin caused 39% inhibition at the optimum pH and temperature.