Abstract
Random Amplified Polymorphic DNA (RAPD) markers and Inter Simple Sequence Repeat (ISSR) markers were used for genetically characterizing of 11 Egyptian wheat genotypes. 8 RAPD and 8 ISSR markers were used to assessment the genetic diversity. RAPD analysis revealed that 60 out of 107 bands (49.45%) were polymorphic while ISSR analysis revealed that 75 out of 96 bands (60.38%) were polymorphic. The UPGMA dendrogram generated using the similarity matrix produced from the banding patterns obtained with RAPD and ISSR markers exhibited different patterns of cluster analysis. In RAPD analysis, the average genetic similarity generated based on RAPD profiling showed 60.4-92.7 % GS between the tested wheat genotypes while in ISSR analysis, showed 66.5-91.1 % GS. Genetic similarity and dissimilarity among genotypes will be useful for genetic variations and can be used to assist efficient selection in wheat breeding programs aiming at achieving sustainability in wheat production in Egypt.