Abstract
Coffee preparation and times of drinking in Arabic peninsula (AP) has ritual folk. Roasting and grinding levels of coffee beans, and additives vary between different parts in AP. the simplest form of Arabic-coffee adds cardamom and saffron to the roasted, ground and boiled coffee; however, the most complex form has more ingredients as ginger, cloves, and ajwain upon desire. Our study addressing the assessment of different forms of Arabic-coffee and their separate ingredients on testis-histology and mRNA expression level of some fertility genes in experimental animals. Fifty-four male albino-rats were divided into 9 groups (6 rats/group). One control group, 5 groups were separately treated with 5 usual Arabic-coffee ingredients, group was treated with coffee and other 2 groups were treated with 2 different Arabic coffee mix as folk recipes. Samples were administrated for 0, 15 and 30 days. The study resulted in non-significant down-regulation in mRNA expression of fertility genes (Testin, StAR and FSHR) However, no histological changes were recorded among the control group rats (CN) and the other treated groups, although there were statistically non-significant differences in between. On conclusion, the usual drinking of roasted Arabic-coffee may be non-effective on testis histology and molecular markers of fertility.