Abstract
Leaf samples from Malva parviflora plants exhibiting vein yellowing and chlorosis were collected during 2013-14 from Lahore, Pakistan and analysed for the presence of begomovirus and associated DNA satellites through PCR and subsequent sequencing. The identified full-length begomovirus genome shared maximum nucleotide (nt) sequence identity at 92.5% with Hollyhock leaf curl virus (HoLCV), representing a new strain, which was tentatively designated as HoLCV-Mal. The betasatellite shared maximum nt sequence identity (92.8%) with Kenaf leaf curl betasatellite (KLCuB) and represents a new isolate of KLCuB. The two identified alphasatellites were 86% identical to each other and shared maximum nt sequence identities at 94.3 and 93.2% with Ageratum conyzoides symptomless alphasatellite (ACSLA) and Ageratum yellow vein India alphasatellite (AYVIA), respectively. Thus, these are the new isolates of ACSLA and AYVIA identified from M. parviflora in Pakistan. To the best of our knowledge, this is the first report of a complete monopartite begomovirus complex from M. parviflora in Pakistan.