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Molecular diagnostics and phylogenetic analysis of 'Candidatus phytoplasma asteris' (16Srl-Aster yellow group) infecting banana (Musa spp.)
Journal article   Open access

Molecular diagnostics and phylogenetic analysis of 'Candidatus phytoplasma asteris' (16Srl-Aster yellow group) infecting banana (Musa spp.)

Braj Raj Singh, Aminuddin, Abdulaziz A. Al-Khedhairy, Fahad Al-Qurainy and Javed Musarrat
African journal of biotechnology, Vol.8(21), pp.5819-5824
02/11/2009

Abstract

Biotechnology & Applied Microbiology Life Sciences & Biomedicine Science & Technology
The presence of phytoplasma in diseased banana plants exhibiting acute stunting symptoms was detected by the polymerase chain reaction using a primer set specific for the 16SrRNA gene of phytoplasma. The amplified DNA fragments of 1500 bp were cloned in pGEMT-Easy plasmid cloning vector and sequenced. The BLASTN and phylogenetic analyses revealed the infecting agents to be the closely related members of the 'Candidatus phytoplasma asteris' (16Sr l-Aster yellow) group.
url
https://doi.org/10.5897/ajb09.1046View
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