Abstract
The Kastela Bay (Croatia) is known, by the use of Fast micromethod, as an area with the highest level of genotoxic agents along the Adriatic Sea. The genotoxic impacts in Kastela Bay and the neighbouring Trogir Bay using micronucleus test and Comet assay with mussel (Mytilus galloprovincialis) haemocytes were assesed during 2003 and 2004. This location received effluents from various industries (brewery, cement plant, etc.), ports (oil and general port), the Split shipyard, Vranjic shipworks along with domestic sewage and agricultural discharge that enters the bay without any treatment. In the meantime, a lot of industries were closed and the input of pollutants were discontinued. The tested mussels from the 2003-2004 confirmed that mussels from Kastela Bay were affected by genotoxic contaminants. The aim of our study was to assess genotoxic impacts on Mytilus galloprovincialis in the same area after six years using Comet Assay test. Our survey showed decreses of genotoxic damage on mussel haemocytes.