Abstract
The present work is aimed to develop and validate a method for the determination of cefpirome from bulk drug and its application in cefpirome–antioxidant interactions. Separation was achieved on a Mediterranea C18 (5 μm, 25 × 0.46 mm) column, mobile phase consisting of methanol:water (30:70, v/v). Mobile phase was pumped at a flow rate of 1 ml min
−1
using isocratic pump system and chromatograms were recorded at 265 nm. Linearity was studied up to 50 μg ml
−1
with correlation coefficient (
r
2
) of 0.9999. The limits of detection (LOD) and quantification (LOQ) were 0.03 and 009 μg ml
−1
, respectively. The intraday and inter-day precision of the method was less than 2% and accuracy was between 98.85 and 100.16%. The method was applied to investigate the interaction of cefpirome with antioxidants (vitamin A, C, E, and sodium metabisulfite). These interactions were evaluated at 37°C for half an hour. The samples were then analyzed by RP-HPLC. The results showed that after interaction with antioxidants, availability of cefpirome was decreased to 58.97, 49.85, 50.51, and 50.15% in the presence of vitamin A, C, E, and sodium metabisulfite, respectively. On the basis of interaction results, it can be concluded that whenever coadministration of cefpirome is required with antioxidants, a proper interval should be given in order to avoid interactions.