Abstract
Here we describe a toolkit for the production of fluorescently tagged proteins in
the
C. elegans
germline and early embryo using Mos1-mediated
single copy insertion (MosSCI) transformation. We have generated promoter and
3′UTR fusions to sequences of different fluorescent proteins yielding
constructs for germline expression that are compatible with MosSCI MultiSite
Gateway vectors. These vectors allow tagged transgene constructs to be inserted
as single copies into known sites in the
C. elegans
genome
using MosSCI. We also show that two
C. elegans
heat shock
promoters (
Phsp-16.2
and
Phsp-16.41
) can be
used to induce transgene expression in the germline when inserted via MosSCI
transformation. This flexible set of new vectors, available to the research
community in a plasmid repository, should facilitate research focused on the
C. elegans
germline and early embryo.