Abstract
The present work aimed to find out a novel agent for green synthesized zinc oxide nanoparticles (ZnONPs) from the fresh fruity bodies of edible mushroom (Hypsizygus ulmarius), and it was characterized by UV-Vis, XRD, HR-TEM, SEM, FT-IR, EDX, PSA, and Zeta potential analysis. UV-Vis spectra result reflects a typical absorption peak at 260 nm due to their significant excitation binding energy at room temperature. The chemical bond formation from the zinc oxide was confirmed by FT-IR analysis. XRD results revealed the hexagonal structure, and SEM analyses reflected the spherical shape ZnONPs with an average size of 27.10 nm. The outcome of the EDX spectrum confirmed the high purity of synthesized ZnONPs, and the zeta potential value indicates the aggregation of particles with good quality. The Hu-ZnONPs expressed a better larval toxicity effect against Culex quinquefasciatus (IVth instar larvae) with the least LC50 and LC90 values (21.52, 121.70 mg/L) after 24 h treatment. For the antibacterial assay, the maximum growth inhibition zone was recorded in S. aureus (14.2 +/- 0.2), E. coli (10.1 +/- 0.3), and K. pneumoniae (9.0 +/- 0.1 mm). The anticancer assay results expressed a significant level of toxic effects (LC50 values 29.07 mu g/ml) of Hu-ZnONPs against the MCF-7 breast cancer cells, even at a very low dose. The study's findings suggest that the H. ulmarius can biosynthesize ZnONPs and could be an alternative biomedical agent for future therapeutic applications.