Abstract
Nanotechnology finds immense application in drug development and its targeted delivery. The growth and biofilm inhibitory potential of silver nanoparticles (AgNPs), synthesized by the ethanomedicinal herb Andrographis paniculata antibiofilm extract against cystic fibrosis, isolate Pseudomonas aeruginosa by using the biofilm specific methods carried out. AgNps were synthesized by a rapid biological method using A. paniculata antibiofilm extract as a reducing agent. UV-Vis spectroscopy and TEM (Transmission electron microscopy) analysis was carried out to confirm and characterize AgNPs. Phytochemical constituents were determined by GC-MS analysis. The dose dependent antibacterial activity based on the well diffusion and modified quantitative spectrophotometric microtiter plate method were used for in vitro anti-biofilm assay. The structure of GC-MS identified phytochemicals exposed the silver reducing diverse functional groups availability. The synthesized particles were 50nm diameter spheres and their thin faint exotic material layer due to phytochemicals interaction, likely explains the stability of AgNPs. AgNPs were found to impede P. aeruginosa exopolysaccharide and biofilm synthesis. Synthesised AgNPs treatment resulted in more than 90% biofilm formation inhibition. The in vitro biofilm inhibition demonstrated the therapeutic potential of A. paniculata antibiofilm activity and their phytochemical drug lead's novel application which may be a surrogate to antibiotics.