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Nano-mole scale sequential signal assignment by H-1-detected protein solid-state NMR
Journal article   Peer reviewed

Nano-mole scale sequential signal assignment by H-1-detected protein solid-state NMR

Songlin Wang, Sudhakar Parthasarathy, Yiling Xiao, Yusuke Nishiyama, Fei Long, Isamu Matsuda, Yuki Endo, Takahiro Nemoto, Kazuo Yamauchi, Tetsuo Asakura, …
Chemical communications (Cambridge, England), Vol.51(81), pp.15055-15058
01/01/2015
DOI: https://doi.org/10.1039/c5cc04618a
PMID: 26317132

Abstract

Chemistry Chemistry, Multidisciplinary Physical Sciences Science & Technology
We present a 3D H-1-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10-100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at similar to 80 kHz by a novel spectral-editing method, which permits drastic spectral simplification. The approach offers similar to 110 fold time saving over a traditional 3D C-13-detected SSNMR approach.

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