Abstract
The need for the bacterial proteinase is rapidly growing, urging to catch a lowcost medium for the microbial fermentation, nanoparticles can play a vital role in this respect. The proteinase of Talaromyces purpureogenus was produced on the tubers of Helianthus tuberosus that also operated as solid support for the fermentation process. The interface amongst nitrogen sources (NH4Cl and yeast extract) was investigated, applying the statistical modeling of central composite design under solid-state fermentation. The optimum medium for proteinase secretion was stimulated by 979.82 mg NH4Cl and 437.68 mg yeast extract per 100 g substrate, yielding 108.15 U/g tubers. Using Plackett-Burman experimental design, the nanoparticles Co, Ni and Fe were assessed as inducers for proteinase stimulants. Co nanoparticles (5 ppm) were the greatest in both proteinase production by the fungus as well as an inducer of the proteolysis process by the enzyme when using faba bean straw as a proteinaceous substrate in the reaction mixture, liberating the extreme quantity of amino acids, compared with the lack of the nanoparticles. The findings suggest the incorporation of Co nanoparticles in both the proteinase fabrication process and during the degradation of proteinaceous materials induce proteinase catalyst. This approach could be extended to modulate the productivity and activity of similar biomolecules.