Abstract
The present study intended to investigate the formulation approaches to develop biodegradable biomass polymeric nanoparticles for delivery of noscapine (NoS) to exploit its cytotoxic potential. The formulations were developed using NoS chitosan nanoparticles (Chi-NPs) with two different coatings of alginate (Alg-Chi-NP) and folic acid (FA-Chi-NPs). The prepared formulation were studied the physiochemical properties of Fourier transform infrared spectrophotometric (FTIR) analysis, differential scanning calorimetric (DSC) analysis, thermogravimetric (TG) analysis and powder X-ray diffraction (PXRD) analysis and drug loading (DL), particle size (PS), and entrapment efficiency (EE), and in vitro release of the drug formulation favor the commercial viability of the prepared NPs. Using natural biodegradable polymers, FA-Chi-NPs was found to best with 220 nm PS and 65% EE. Best selected formulation from natural biodegradable polymers, i.e., FA-Chi-NPs were studied for their in vitro anticancer study using MTT assay in MCF-7 cells and HeLa cells. The FA-Chi-NPs showed lower IC50 values in both in MCF-7 cells (26.8 mu M.mL(-1)) and HeLa (11.7 mu M.mL(-1)) cells as compare to NoS free drug. Both the lyophilized Alg-Chi-NP and FA-Chi-NPs was found to be stable for 6 months at 2-8 degrees C, ambient conditions and in GI fluids. In general, the findings of this study demonstrate that FA-Chi-NPs might be established as a successful carrier for NoS to exploit its anticancer efficacy and may provide valuable addition to its existing therapeutic uses.