Abstract
Five new monomethine cyanine dyes have been prepared and spectroscopically characterized. The acetamido dyes present to be promising tools for DNA analysis at low levels of concentration when UV light is required for visualization, overcoming the use of the mutagenic EtBr stain or radioactivity for detecting low levels of DNA.
Some new monomethine cyanine dyes derived from quinoline and benzothiazole have been prepared and characterized by
1H and
13C NMR, FTIR, FABHRMS, and visible spectroscopy. The dyes containing amino and acetamido groups were conveniently synthesized by the condensation of two
p-toluenesulfonate heterocyclic quaternary salts and were obtained in the forms of iodide, bromide, and tosylate counteranions. These dyes were compared to ethidium bromide as stains for DNA in electrophoretic gels. The overall results obtained for the sensitivity of these dyes suggest the suitability of acetamido moiety over the amine one and bromide as the counteranion when compared with iodide and tosylate, with a similar capacity of DNA detection in relation to the ethidium bromide stain over the concentration range of 1–3
ng.