Abstract
β-Sitosterol-3-O-(3Z)-pentacosenoate (1), 5α-pregna-3β-acetoxy-12β,16β-diol-20-one (2), and echinoclathriamide ((R)-2′-hydroxy-N-((2S,3S,4R)-1,3,4-trihydroxy-19-methylicosan-2-yl)heptadecanamide) (3) were isolated from the Red Sea sponge Echinoclathria gibbosa.
•The EtOAc fraction of the sponge Echinoclathria gibbosa was investigated.•Three new and two known compounds were isolated.•Their structures were established by different spectroscopic analyses.•Their in vitro growth inhibitory activity was estimated.•Antimicrobial, anti-inflammatory, antipyretic, and hepatoprotective activities were evaluated.
Three new compounds; β-sitosterol-3-O-(3Z)-pentacosenoate (1), 5α-pregna-3β-acetoxy-12β,16β-diol-20-one (2), and echinoclathriamide ((R)-2′-hydroxy-N-((2S,3S,4R)-1,3,4-trihydroxy-19-methylicosan-2-yl)heptadecanamide) (3), together with two known compounds; thymine (4) and uracil (5) were isolated from the EtOAc fraction of the Red Sea sponge Echinoclathria gibbosa. Their structures were unambiguously established on the basis of 1D and 2D NMR spectroscopy, in addition to mass spectrometry. The total MeOH extract (TME) and its fractions were evaluated for their antimicrobial, anti-inflammatory, antipyretic, and hepato-protective activities. The in vitro growth inhibitory activity of the isolated compounds was evaluated against three human cancer cell lines including the A549 non-small cell lung cancer (NSCLC), U373 glioblastoma (GBM), and PC-3 prostate cancer cell lines. Compound 1 showed weak activity against the three cancer cell lines.