Abstract
Biogas is one of the practical vitality sources of energy that can be delivered from numerous sorts of biomass. Biogas production from fresh digestate bacterial crude culture; Providencia alcalifaciens and Alcaligenes aquatilis isolated from municipal wastewater treatment plants located in Port Said, Egypt and identified with 16S rRNA; amended with aquatic macrophytes; Pistia stratiotes and Potamogeton pectinatus, collected from some irrigation canals in Port Said, Egypt as a substrate in the presence of different concentrations of chemically prepared nickel oxide nanoparticles (NiONPs) subjected to anaerobic conditions were successfully investigated. The produced biogas was collected by the water displacement method and subsequently measured using GC/MS analysis. As found in this present work, the optimum NiONPs concentration was 2 ppm for biogas production, where the highest productivity was 42.90 mL and 31.33 mL/3.4 g of P. stratiotes and P. pectinatus with enhancing % 87.91% and 82.38%, respectively compared with the control (without NiONPs) at 30 days of incubation period. Biogas production was slightly greater using P. stratiotes than P. pectinatus particularly with the addition of NiONPs. The present examination may have a noteworthy task in the generation of reasonable biogas amounts utilizing monetary wellsprings of reusing the horrible water system trenches macrophytes and control of environmental defilement issues that may have come about in light of these unfortunate macrophytes.
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