Abstract
The nitrogen use efficiency (NUE) is the ratio of the N uptake by the plant to the total amount of applied N fertilizer. The optimization of NUE is essential for sustainable agriculture, especially in the breeding programs to improve the yield of barley (Hordeum vulgare L.). We investigated the NUE variation in 32 barley genotypes and to examine the response relationship between phenotypic traits and N level to determine the traits essential for the selection of breeding programs to optimize the NUE. The NUE was investigated in 32 barley genotypes for 2 yr under field conditions using three N levels (60, 120, and 180 kg N ha(-1)). Growth, physiological and agronomic traits, and N efficiency-related parameters were determined. The parameters investigated were significantly affected by N application and its interactions with traits. Plant height, biological yield, spike length, and grain number (GN) were increased at higher N levels, but the 1,000-grain weight decreased. The lowest grain yield was 2,000.7 kg ha(-1) at 60 kg N ha(-1), and the highest was 8,607.9 kg ha(-1) at 120 kg N ha(-1). An increase in the N rate from 60 to 120 kg N ha(-1) was associated with a higher total chlorophyll content (CHLSPAD) (from 19.04 to 23.13), but no significant change in CHL was seen at 180 kg N ha(-1). The agronomic nitrogen use efficiency (ANUE), fertilizer N recovery efficiency and the nitrogen utilization efficiency were both reduced with an increase of the N application rate. A path analysis revealed that GN was the most essential trait for NUE breeding program selection due to its high direct effect (0.72) on ANUE. A linear discriminant analysis showed that CHL at 60 d after sowing was the best trait for distinguishing low from high N levels.