Abstract
The prevalence of risk factors of chronic kidney disease in Saudi Arabia has augmented an already serious public health problem, therefore, determination of genetic variants associated with the risk of the disease presents potential screening tools that help reducing the incidence rates and promote effective disease management.
The aim of the present study is to determine the association of
UMOD
and
MYH9
genetic variants with the risk of non-diabetic end-stage renal disease (ESRD) in the Saudi population.
Two single nucleotide polymorphisms (SNP), rs12917707 in gene
UMOD
and rs4821480 in gene
MYH9
were genotyped in 154 non-diabetic ESRD Saudi patients and 123 age-matched healthy controls using Primers and Polymerase chain reaction conditions (PCR), Sanger sequencing, and TaqMan Pre-designed SNP Genotyping Assay. The association of these genetic variants with the risk of the disease and other renal function determinants was assessed using statistical tools such as logistic regression and One-way Analysis of Variance tests.
The genotypic frequency of the two SNPs showed no deviation from Hardy–Weinberg equilibrium, the minor allele frequency of UMOD SNP was 0.13 and
MYH9
SNP was 0.08. rs4821480 in
MYH9
was significantly associated with the risk of non-diabetic ESRD (OR = 3.86; 95%CI: 1.38–10.82,
P
value .010), while, rs12917707 showed lack of significant association with the disease,
P
value .380. and neither of the 2 SNPs showed any association with the renal function determinants, serum albumin, and alkaline phosphatase enzyme.