Abstract
A simple, efficient, reliable and cost-effective method for isolation of total genomic DNA from fungi, suitable for polymerase chain reaction (PCR) amplification and other molecular applications was described. The main advantages of the method are: (1) does not require the use of liquid nitrogen for preparation of fungi DNA; (2) the mycelium is directly recovered from Petri-dish cultures; (3) the quality and quantity of DNA obtained are suitable for molecular assays; (4) the technique is rapid and relatively easy to perform; (5) it can be applied to filamentous fungi from soil as well as from a fungi from other environmental sources; and (6) it does not require the use of expensive and specialized equipment or hazardous reagents. This method does not require liquid nitrogen for fixation, grinding or storage at 80 C, making it advantageous over other common protocols.