Abstract
This paper presents a novel, high sensitive, facile and rapid spectrofluorimetric method for the direct assessment of glucose in human serum samples. The strong fluorescence intensity of pyronin Y (PY) is found to be selectively quenched substantially by adding solutions of glucose. Therefore, a new quantitative method to determine glucose could be developed. The noticeable and specific quenching effect of glucose on PY emission could be attributed to the increase in the nonradiative interactions as a result of a charge-transfer excited state with the location of the positive charge at the xanthenes moiety. Various parameters namely, the best working solvent, the proper pH medium in addition to the concentration of PY fluorophore corresponding to maximum fluorescence intensity were closely investigated. Under optimal conditions, the standard curve was linear in the glucose concentration ranges from 5.0×10−9 to 1.0×10−6molL−1 (r=0.994). The detection limit (S:N=3) (LOD) is 2.9×10−9molL−1. Validation of this approach was done by examining glucose concentration in serum samples of twenty patient and ten health donors. Achieved recovery reached 99.63–100.43% and 99.57–100.70%, respectively, in good agreement with those provided from hospital using traditional method. The relative standard deviations (RSD) were estimated between 0.00–0.78% and 0.15–1.91%, respectively.
► A novel spectrofluorimetric determination of glucose is established. ► It added a novel function of pyronin Y(PY) as a viable glucose fluorimetric probe. ► The method reveals glucose selective and substantial quenching of PY fluorescence. ► The method is rapid, facile, sensitive, interference free and reproducible.