Abstract
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•Spectrofluorimetric determination of brigatinib via ion-pair complex formation using eosin Y.•Optimization of experimental conditions that affect the fluorescence of the formed ion pair complex.•Simplicity, cost-effectiveness, high sensitivity of the current methodology over the reported LC/MS method.
The developed spectrofluorimetric method was successfully applied to the analysis of brigatinib (BRG) in its bulk powder form, and human urine sample.
It is based on the investigation of the fluorescence spectrum behavior of the BRG-eosin Y complex. The relative fluorescence intensity (RFI) was recorded at 560 nm after excitation at 480 nm. The principle of the proposed method was thoroughly explained. All experimental parameters affecting method development were optimized. Moreover, the obtained results were fully discussed and statistically analyzed. The molar ratio method was applied to study the stoichiometric relationship between BRG and eosin Y complex. The method revealed a ratio of 1:3 for BRG-eosin Y afforded the highest RFI. The developed method was validated over the concentration range of 62.5–4000 ng mL−1. The results were compared positively with the reported method.