Abstract
One-step approach of cholesterol biosensor was fabricated onto smart micro-chips based on cholesterol oxidase (ChOx) co-immobilized thioglycolic acid self-assembled monolayer (TGA-SAM) for biomedical applications. The selective cholesterol biosensor was investigated with modified tiny micro-chip (Au/SAM/ChOx) by the facile and reliable cyclic voltammetric (CV) method in a K3Fe(CN)(6)/phosphate buffer (PB) system. The modified micro-chip displayed a large dynamic range (1.0 nmol L-1 to 1.0 mmol L-1), lower detection limit (similar to 0.49 nmol L-1, based on SIN similar to 3), higher sensitivity (similar to 93.75 mu A mu mol L-2 cm(-2)), good linearity (correlation coefficient r(2), 0.9995), lower sample volume (<50.0 mu l), and good stability as well as reproducibility. The Au/TGA system was implemented for a facile and simple approach to the immobilization of ChOx onto micro-chip, which can offer analytical access to a large group of enzymes for a wide range of bio-molecule applications in health-care and biomedical fields. This integrated microchip provides a promising low-cost platform for the sensitive and rapid detection of biomolecules using miniatured samples. (C) 2015 Elsevier B.V. All rights reserved.