One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports

Journal article

Peer reviewed

Cesar Mateo, Gloria Fernández-Lorente, Estrella Cortés, José L. Garcia, Roberto Fernández-Lafuente and José M. Guisan

Biotechnology and bioengineering, Vol.76(3), pp.269-276

11/2001

PMID: 11668463

Abstract

glutaryl acylase

immobilized metal chelate affinity chromatography: epoxy supports

multipoint covalent attachment

selective immobilization of enzymes

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Title: One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports
Creators - without role: Cesar Mateo - Instituto de Catálisis y Petroleoquímica
Gloria Fernández-Lorente - Instituto de Catálisis y Petroleoquímica
Estrella Cortés - Centro de Investigaciones Biológicas Margarita Salas
José L. Garcia - CIUDAD
Roberto Fernández-Lafuente - Instituto de Catálisis y Petroleoquímica
José M. Guisan - Instituto de Catálisis y Petroleoquímica
Publication Details: Biotechnology and bioengineering, Vol.76(3), pp.269-276
Publisher: John Wiley & Sons, Inc
Number of pages: 8
Identifiers: 9938089608331
Academic Unit: King Abdulaziz University
Language: English
Resource Type: Journal article