Abstract
To avoid the existing limitations of the PCA process for the precipitation of proteins and polar polymers a new approach based on the use of water to modify the phase behaviour of the system DMSO–CO
2 was developed. The addition of water to the DMSO displaces the mixture critical pressure of the solution–CO
2 system to higher pressures and produces a new operating region in which the PCA process can be carried out. Following this approach, it was possible to develop a process to produce 1–10
μm particles of
N-trimethylchitosan chloride (TMC) suitable for inhalation. Encapsulation of proteins in TMC using this technique could also be successfully achieved.