Abstract
Bacteriocins are nowadays recognized as good biopreservatives that are able to contain microbial contaminant development in several food products. In this study, the goat milk isolate Lactobacillus brevis NS01 brevicin activity on common foodborne pathogenic organisms and its characterization, stability, and statistical response surface methodology (RSM) optimization of media components for enhanced production were determined. Its adsorption onto common stabilizing agent silica and applicability in apple juice preservation were also determined. The L. brevis NS01 produced 3.2 kDa common foodborne pathogen inhibitory brevicin having more polar (68.6 %) than non-polar (30.8 %) amino acids. Optimization of growth conditions by RSM demonstrated that glucose, sodium acetate, and triammonium sulfate concentration of 33.41, 8.36, and 2.22 g/L, respectively, increased its production. Brevicin activity was constant over a broad range of pH (3-8) and temperature (20-100 A degrees C) and reached a maximum at pH 6 and 40 A degrees C. Maximum adsorption of brevicin onto silica took place at pH 6-7, 40 A degrees C for 240 min. The carrier silica exhibited significant Fourier transform infrared (FTIR) spectral changes upon brevicin adsorption and presented good biopreservative activity. This study demonstrates the potential large-scale use of brevicin adsorbed silica for shelf life increase and clarification at a same time.