Abstract
Aim: To evaluate the osteogenic activity of NeoMTA Plus using rat mesenchymal stem cells (MSCs). Materials & Methods: Pre-seeded rat MSCs were cocultured with either NeoMTA Plus or ProRoot-MTA and incubated for 1, 3 and 7 days (6 well for each) .The cell growth, viability and proliferation were evaluated with light and scanning electron microscope. The specimens were also subjected to quantitative real time-polymerase chain reaction for osteogenic gene expression. Results: After the treatment with either material, cell viability gradually increased by time, and the cells showed signs of differentiation into osteoblasts. ProRoot-MTA exhibited a significant higher mean value than NeoMTA plus after 7 days. At the third and seventh days, the cells treated with both materials exhibited deep alkaline phosphatase staining more than control untreated cells. Upregulation of osteogenic gene expression including bone morphogenetic-2, alkaline phosphatase, bone sialoprotein, osteopontin and osteocalcin was observed with both materials. Conclusion: Both NeoMTA Plus and ProRoot-MTA had osteogenic activity when used as pulp and endodontic repair material.