Abstract
Photohemolysis of erythrocytes using five lasers of different wavelengths and two photosensitizers viz. acridine orange and fluorescein was studied. The per cent hemolysis in the presence of laser and sensitizer was higher when compared to laser alone. Of the two sensitizers, acridine orange had a higher effect in photomodification of cells than fluorescein. Hemolysis was found to increase towards shorter wavelengths. Per cent hemolysis depends on post incubation time, indicating the photochemical nature of the damage due to the delayed photo-oxidation via the cross-linking between biomolecules.