Abstract
The objective of our study was to formulate different types of liposomes containing Propranolol HCl (Pr), e.g. Multilamellar Vesicles (MLVs), Large Unilamellar Vesicles (LUVs), and Small Unilamillar Vesicles (SUVs) using various phospholipids. MLVs were prepared by freezing thawing method. The freeze-thawing method was chosen because of its simplicity and absence of organic solvents. LUVs were prepared by extrusion of the prepared MLVs through polycarbonate membrane. SUVs were prepared by sonication of the prepared MLVs using probe tip sonicator. The amount of Pr entrapped was determined using HPLC and the entrapment efficiency of the prepared MLVs was calculated. Among the liposome formulations, Phospholipone 90H liposomes showed the highest encapsulation percentage (90.11 %) compared to distearoyl Phosphatidylcholine (DSPC) (88.31%), mixture of distearoyl Phosphatidylcholine (DSPC) and dimyristoyl phosphatidyl choline (DMPC) in a ratio of 1: 1 (71.33%) and dimyristoyl phosphatidyl choline (DMPC) (45.68%). The particle size was analyzed by Laser Diffraction Analyzer (LDA). It was found that the particle size was 6.590, 0613 and 0.453 nm for MLVs, LUVs and SUVs respectively. The in vitro release of Pr from the different liposomes was determined using dialysis method. It was found that the release of Pr from the prepared liposomes depends not only on the type of liposomes but also on the fatty chain length.