Abstract
A novel strain of the genus Paenibacillus, KFC91(T), was isolated from the Kafni glacier of the Himalayas. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KFC91T clustered with Paenibacillus antarcticus LMG 22078(T) (98.9%) and Paenibacillus macquariensis LMG 6935(T) (98.7%). The cell wall peptidoglycan contained meso-diaminopimelic acid as the diamino acid, anteiso-C-15:0 as the predominant fatty acid and MK-7 as the major isoprenoid quinone. Based on the phylogenetic analysis, chemotaxonomic characteristics and other phenotypic traits, strain KFC91(T) was assigned to the genus Paenibacillus. DNA-DNA hybridization experiments of KFC91(T) with P. antarcticus and P. macquariensis showed reassociation values of 39 and 52%, respectively. Thus, it is proposed that strain KFC91(T) should be assigned the status of a novel species of the genus Paenibacillus and the name proposed is Paenibacillus glacialis sp. nov., with KFC91(T) (=NCCB 100252(T) =DSM 22343(T)) as the type strain.