Abstract
A novel xylanolytic and cellulolytic strain, BL9(T), was isolated from leaves of the Bamboo plants maintained at the Tamil Nadu Agricultural University Campus, Coimbatore, India. On the basis of the results of 16S rRNA gene analysis, it was determined to be phylogenetically close to the type strains of Paenibacillus amylolyticus NRRL NRS-290(T) (98.3 %), Paenibacillus barcinonensis BP-23(T) (98.1 %), Paenibacillus tundrae A10b(T) (98.0 %) and Paenibacillus xylanexedens B22a(T) (97.6 %). The strain stained Gram variable and was aerobic, motile and catalase- and oxidase-positive, with rod-shaped cells. Based upon the genome sequence, the average nucleotide identity with the related species ranged from 66% to 72 %, and the digital DNA-DNA hybridization value ranged from 13% to 27 %. The DNA G+C content was 45.6 mol%, meso-diaminopimelic acid was identified as the predominant component of the cell wall, and MK-7 was the only menaquinone in cell membranes. The whole-cell fatty acid profile included C-16 : 0, iso-C-14 : 0, anteiso-C-15 : 0 and iso-C-16 : 0. The polar lipids identified were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine and diphosphtidylglycerol. On the basis of these polyphasic taxonomic traits, BL9(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus polysaccharolyticus sp. nov. is proposed. The type strain is BL9(T) (=NBRC 105191(T)=ICMP 17623(T)).