"Perfect" designer chromosome V and behavior of a ring derivative

Ze-Xiong Xie; Bing-Zhi Li; Leslie A Mitchell; Yi Wu; Xin Qi; Zhu Jin; Bin Jia; Xia Wang; Bo-Xuan Zeng; Hui-Min Liu; Xiao-Le Wu; Qi Feng; Wen-Zheng Zhang; Wei Liu; Ming-Zhu Ding; Xia Li; Guang-Rong Zhao; Jian-Jun Qiao; Jing-Sheng Cheng; Meng Zhao; Zheng Kuang; Xuya Wang; J Andrew Martin; Giovanni Stracquadanio; Kun Yang; Xue Bai; Juan Zhao; Meng-Long Hu; Qiu-Hui Lin; Wen-Qian Zhang; Ming-Hua Shen; Si Chen; Wan Su; En-Xu Wang; Rui Guo; Fang Zhai; Xue-Jiao Guo; Hao-Xing Du; Jia-Qing Zhu; Tian-Qing Song; Jun-Jun Dai; Fei-Fei Li; Guo-Zhen Jiang; Shi-Lei Han; Shi-Yang Liu; Zhi-Chao Yu; Xiao-Na Yang; Ken Chen; Cheng Hu; Da-Shuai Li; Nan Jia; Yue Liu; Lin-Ting Wang; Su Wang; Xiao-Tong Wei; Mei-Qing Fu; Lan-Meng Qu; Si-Yu Xin; Ting Liu; Kai-Ren Tian; Xue-Nan Li; Jin-Hua Zhang; Li-Xiang Song; Jin-Gui Liu; Jia-Fei Lv; Hang Xu; Ran Tao; Yan Wang; Ting-Ting Zhang; Ye-Xuan Deng; Yi-Ran Wang; Ting Li; Guang-Xin Ye; Xiao-Ran Xu; Zheng-Bao Xia; Wei Zhang; Shi-Lan Yang; Yi-Lin Liu; Wen-Qi Ding; Zhen-Ning Liu; Jun-Qi Zhu; Ning-Zhi Liu; Roy Walker; Yisha Luo; Yun Wang; Yue Shen; Huanming Yang; Yizhi Cai; Ping-Sheng Ma; Chun-Ting Zhang; Joel S Bader; Jef D Boeke; Ying-Jin Yuan

doi:10.1126/science.aaf4704

Back

"Perfect" designer chromosome V and behavior of a ring derivative

Journal article

Open access

Peer reviewed

"Perfect" designer chromosome V and behavior of a ring derivative

Ze-Xiong Xie, Bing-Zhi Li, Leslie A Mitchell, Yi Wu, Xin Qi, Zhu Jin, Bin Jia, Xia Wang, Bo-Xuan Zeng, Hui-Min Liu, …

Science (American Association for the Advancement of Science), Vol.355(6329), pp.1046-1046

10/03/2017

DOI: https://doi.org/10.1126/science.aaf4704

PMID: 28280151

Abstract

Bacterial Proteins

Chromosomes, Artificial, Yeast - chemistry

Chromosomes, Artificial, Yeast - genetics

Clustered Regularly Interspaced Short Palindromic Repeats

CRISPR-Associated Protein 9

Endonucleases

Gene Editing

Gene Rearrangement

Genome, Fungal

Meiosis

Models, Genetic

Saccharomyces cerevisiae - cytology

Saccharomyces cerevisiae - genetics

Synthetic Biology - methods

Transformation, Genetic

Perfect matching of an assembled physical sequence to a specified designed sequence is crucial to verify design principles in genome synthesis. We designed and de novo synthesized 536,024-base pair chromosome synV in the "Build-A-Genome China" course. We corrected an initial isolate of synV to perfectly match the designed sequence using integrative cotransformation and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated editing in 22 steps; synV strains exhibit high fitness under a variety of culture conditions, compared with that of wild-type V strains. A ring synV derivative was constructed, which is fully functional in under all conditions tested and exhibits lower spore viability during meiosis. Ring synV chromosome can extends Sc2.0 design principles and provides a model with which to study genomic rearrangement, ring chromosome evolution, and human ring chromosome disorders.

Files and links (1)

url

https://doi.org/10.1126/science.aaf4704View

Published (Version of record) Open

Metrics

1 Record Views

See more details

Details

Title: "Perfect" designer chromosome V and behavior of a ring derivative
Creators - without role: Ze-Xiong Xie - Tianjin University
Bing-Zhi Li - Tianjin University
Leslie A Mitchell - NYU Langone Health
Yi Wu - Tianjin University
Xin Qi - Tianjin University
Zhu Jin - Tianjin University
Bin Jia - Tianjin University
Xia Wang - Tianjin University
Bo-Xuan Zeng - Tianjin University
Hui-Min Liu - Tianjin University
Xiao-Le Wu - Tianjin University
Qi Feng - Tianjin University
Wen-Zheng Zhang - Tianjin University
Wei Liu - Tianjin University
Ming-Zhu Ding - Tianjin University
Xia Li - Tianjin University
Guang-Rong Zhao - Tianjin University
Jian-Jun Qiao - Tianjin University
Jing-Sheng Cheng - Tianjin University
Meng Zhao - Tianjin University
Zheng Kuang - NYU Langone Health
Xuya Wang - NYU Langone Health
J Andrew Martin - NYU Langone Health
Giovanni Stracquadanio - High Throughput Biology
Kun Yang - High Throughput Biology
Xue Bai - Tianjin University
Juan Zhao - Tianjin University
Meng-Long Hu - Tianjin University
Qiu-Hui Lin - Tianjin University
Wen-Qian Zhang - Tianjin University
Ming-Hua Shen - Tianjin University
Si Chen - Tianjin University
Wan Su - Tianjin University
En-Xu Wang - Tianjin University
Rui Guo - Tianjin University
Fang Zhai - Tianjin University
Xue-Jiao Guo - Tianjin University
Hao-Xing Du - Tianjin University
Jia-Qing Zhu - Tianjin University
Tian-Qing Song - Tianjin University
Jun-Jun Dai - Tianjin University
Fei-Fei Li - Tianjin University
Guo-Zhen Jiang - Tianjin University
Shi-Lei Han - Tianjin University
Shi-Yang Liu - Tianjin University
Zhi-Chao Yu - Tianjin University
Xiao-Na Yang - Tianjin University
Ken Chen - Tianjin University
Cheng Hu - Tianjin University
Da-Shuai Li - Tianjin University
Nan Jia - Tianjin University
Yue Liu - Tianjin University
Lin-Ting Wang - Tianjin University
Su Wang - Tianjin University
Xiao-Tong Wei - Tianjin University
Mei-Qing Fu - Tianjin University
Lan-Meng Qu - Tianjin University
Si-Yu Xin - Tianjin University
Ting Liu - Tianjin University
Kai-Ren Tian - Tianjin University
Xue-Nan Li - Tianjin University
Jin-Hua Zhang - Tianjin University
Li-Xiang Song - Tianjin University
Jin-Gui Liu - Tianjin University
Jia-Fei Lv - Tianjin University
Hang Xu - Tianjin University
Ran Tao - Tianjin University
Yan Wang - Tianjin University
Ting-Ting Zhang - Tianjin University
Ye-Xuan Deng - Tianjin University
Yi-Ran Wang - Tianjin University
Ting Li - Tianjin University
Guang-Xin Ye - Tianjin University
Xiao-Ran Xu - Tianjin University
Zheng-Bao Xia - Tianjin University
Wei Zhang - Tianjin University
Shi-Lan Yang - Tianjin University
Yi-Lin Liu - Tianjin University
Wen-Qi Ding - Tianjin University
Zhen-Ning Liu - Tianjin University
Jun-Qi Zhu - Tianjin University
Ning-Zhi Liu - Tianjin University
Roy Walker - University of Edinburgh
Yisha Luo - University of Edinburgh
Yun Wang - BGI Group
Yue Shen - BGI Group
Huanming Yang - BGI Group
Yizhi Cai - University of Edinburgh
Ping-Sheng Ma - Tianjin University
Chun-Ting Zhang - Tianjin University
Joel S Bader - High Throughput Biology
Jef D Boeke - NYU Langone Health
Ying-Jin Yuan - Tianjin University
Publication Details: Science (American Association for the Advancement of Science), Vol.355(6329), pp.1046-1046
Grant note: BB/M005690/1 / Biotechnology and Biological Sciences Research Council
Identifiers: 9942026608331
Academic Unit: King Abdullah University of Science & Technology
Language: English
Resource Type: Journal article