Abstract
Microcystin, a cyanotoxin produced by
Microcystis aeruginosa, lacks potent antibacterial activity. When tested in combination, in vitro, inhibitory values for a range of hydrophobic antibiotics were significantly reduced in the presence of at least 1/20×the minimum inhibitory concentration of microcystin. The degree of inhibition was equivalent to that of a well-characterised permeabilising agent, polymyxin B nonapeptide. The permeabilising ability of sub-inhibitory concentrations of microcystin to affect the envelope of
Escherichia coli was demonstrated by a rapid and sustained reduction in absorbance values of lysozyme-treated cells and by enhanced uptake of crystal violet in microcystin-treated cultures. Direct effects of appropriate concentrations of microcystin on the integrity of bacterial outer and inner membranes were measured by release of specific enzyme markers. Although the exact mechanism for permeabilising
E. coli with microcystin has not been elucidated, the effects were consistent with permeability changes to the enterobacterial outer membrane caused by polymyxin B nonapeptide.