Abstract
Background and Objective: Recently researchers are using bionanotechnology techniques as environmentally safe and cost-less means for processing nanoparticles. This study is designated to document characters of silver nanoparticles synthesized from cold and hot water fruits extract of Solanum incanum and investigating their antimicrobial activity. Materials and Methods: Silver nanoparticles formation from cold and hot water fruits extract of Solanum incanum was characterized by UV-Vis spectral analysis, X-Ray diffraction and scanning electron microscopy. Nanoparticles (NPs) antimicrobial activity had been investigated through Well-diffusion methods. Results: UV-Vis spectroscopy showed broad absorption peaks located at 428.66 nm for NPs of cold water extract and 445.73 nm for hot water extract of S. incanum fruits. NPs of cold water extract showed some agglomerated nanoparticle in the form of nanoclusters, while hot water extract has a spherical shape of a very low dimension. Antimicrobial assay confirmed the bactericidal and candidacidal activity of biosynthesized AgNPs toward Gram-negative bacteria, Gram-positive and Candida albicans. S. aureus is the most sensitive microorganism to the activity of NPs from cold and hot aqueous extracts as the percent differences found to be (21.81%), followed by S. flexneri (18.74%), M. luteus by (11.69%) and C. albican by (11.60%). On the other side, percentage differences between NPs from cold and hot aqueous fruit extracts found to be low on K. oxytoca by (0.783%), followed by P. mirabilis (4.515%) and P. aeruginosa (4.713%). Conclusion: There were obvious differences between the synthesized nanoparticle's cold and hot extract of Solanum incanum fruits with remarkable antimicrobial properties.