Abstract
Bioactive compounds are responsible for biological activities in honey. The botanical and regional sources of honey contribute to the variable concentration of bioactive compounds. This paper reports the analysis of bioactive compounds such as phenolic compounds, vitamin C, total phenolic contents (TPC), radical scavenging activity (RSA), and sugars of five honey samples (Talh, Athel, Sidr, Spring flower, and Langnese) from the ingenious Hail region (Saudi Arabia) using HPLC-RID and DAD. Talh has the highest TPC level of 26.9 mg GAE/100 g, whereas Spring flower has the lowest level of 8.2 mg GAE/100 g. Quercetin levels in all samples ranged from 0.28 to 2.68 mg GAE/100 g. Gallic acid, a phenolic compound, was found in three samples of honey at concentrations ranging from 0.81-1.08 mg/100 g. DPPH radical scavenging activity (RSA) of Talh and Sidr honey sample are found to be high as compared to other samples. The Sidr honey sample had the highest vitamin C content, 2.59 mg/100 g. Fructose and glucose sugar concentrations ranged from 28.35-37.81 g/100 g and 20.21-32.28 g/100 g, respectively, with a higher fructose ratio. Sucrose was not found in any of the five samples. These findings point to the high quality of honey produced in Saudi Arabia's ingenious Hail province, and therefore may contribute in therapeutic use of these types of honey, such as in complementary and alternative medicine.