Abstract
The impact of (100 to 500 Gy) of gamma-irradiation on M-2 plants of three cultivars of soybean and their association with ISSR fingerprinting have been studied. Morphological variation was scored as plant size, roots nodule formation, leaf shape, size and colour, pod shape, seed hilum colour, testa surface texture and testa colour. Ten ISSR primers successfully amplified 137 reliable markers. In general, reduction in polymorphic markers were associated with increased gamma-irradiation doses for the three soybean cultivars. Pearson correlation coefficient indicated that the number of unique and polymorphic markers are generally correlated with the visible variations in some morphological traits which may be regarded as potential mutations. These findings indicate the importance of assessing changes in phenotypic traits, induced by mutagens, and their associated genetic markers.