Abstract
Nanomaterials are basic tool in ongoing research having tremendous clinical, antitumorcal, microbial, non-microbial applications. Due to their unique properties such as high quantum yield, smaller size and tunable emission wavelength over wide spectrum of light, they are front runners
Nano-dependent PDT technique involving nanoparticles (NPs) is simple, biologically safe, biocompatible in absence of UV light, increases endogenous fluorescence, noninvasive, fast with their least permeability in normal cells. But nickel oxide nanoparticles (NiO NPs) having large surface to
volume ratio shows maximum toxicity. Hence they could be utilized as an efficient photosensitizer by providing intrinsic white light leads to the necrosis effect. The main focus of our research is to improve the effectiveness of PDT by using malignant cell line as biological model. In present
experiment, NiO NPs were synthesized by using precipitation technique. After successful growth of mentioned NPs, characterization step had been performed. In the last step, toxicity of NiO will be tested in hepatocellular model (HepG2 cell line). Our main objective was to determine the actual
cell killing effects (via apoptosis and necrosis) and relevant parameters relationship with loss in cell viability using HepG2 as an experimental biological model. After successful investigation of Biotoxicity of HepG2 cellular model the author will be able to quote the protocol for real treatment
of liver cancer patients.