Abstract
In the current study, photodynamic damage in different cell cultures was examined using a pulsed laser light. Two different experiments were performed to analyse the photodynamic damage. For the first one, a stimulated Raman scattering laser has been obtained by exciting DMSO liquid with Nd-YAG laser, second harmonic generation, 532 nm. The resulted SRS wavelength is pulsed 630 nm. 1 ml ALA (200 mu g/ml) was added to cell suspension and keep it for incubated for 4 h then irradiate the suspension with SRS wavelength 630 nm at different light dose 8, 12, 17, 25, 30, 40, 60, 100, 150, 200 mu J for 10 pluses and obtain the cell degradation. We repeat the step above but for 30 pluses. Finally for the second experiment, 1 ml ALA (200 mu g/ml) was added to cell suspension and was incubated for 4 h and then irradiated with Nd-YAG Laser at wavelength 532 nm. Different doses range between 8 up to 200 mu J for 10 pluses only and the cell degradation rate was measured.