Abstract
The current study was conducted to investigate the total phenolic and total flavonoid contents, the antioxidant activity and to clarify the chemical composition of different extracts of different plant organs of Sisymbrium irio L as a comparative study. Sisymbrium irio L is characterized chemically by the presence of various secondary metabolites such as flavonoids, triterpenoids/steroids, tannins, carbohydrates, saponins and alkaloids at different levels in different extracts of plant organs and the absence of cardiac glycosides and Anthraquinones. The antioxidant activity was determined in accordance to 1,1-diphenyl-2-picryl-hydrazyl radical scavenging protocol and using trolox as standard. Moreover, the total phenolic and total flavonoid contents were evaluated using Folin-Ciocalteu reagent. The results showed that the extracts of ethyl acetate, butanol and aqueous of leaves, stems and flowers exhibited a higher antioxidant activity which further confirmed by its higher amounts of phenolic and flavonoid contents among all the tested extracts. These results revealed that the ethyl acetate, butanol and aqueous extracts of different plant organs of Sisymbrium irio L could be used as a potential nutraceutical antioxidant natural product formulation.