Abstract
In the present study, the antifungal activity of wood treated with Pinus halepensis branch n-hexane oily liquid extract (OLE) and Schinus terebinthifolius branch essential oil (EO) was evaluated against the growth of four phytopathogenic fungi-Bipolaris oryzae, Fusarium oxysporum, Fusarium solani, and Rhizoctonia solani. Air-dried wood samples of Pinus roxburghii were autoclaved, and each wood received 100 mu L of the concentrated oils from P. halepensis and S. terebinthifolius. The main compounds identified in S. terebinthifolius branch EO were terpinen-4-ol (18.25%), cis-beta-terpineol (15.60%), gamma-terpinene (12.46%), sabinene (9.83%), alpha-terpinene (8.56%), and 4-thujanol (6.71%), while the main compounds in P. halepensis branch HeO were 2-undecenal (22.25%), 4-hydroxy-10-methyl-3,4,7,8,9,10-hexahydro-2H-oxecin-2-one (8.43%), (Z)-2-decenal (6.88%), nonanal (5.85%), (2E)-2-decenal (4.65%), (E,E)-2,4-decadienal (4.41%), arachidonic acid methyl ester (4.36%), and 2-(7-heptadecynyloxy)tetrahydro-2H-pyran (4.22%). P. halepensis OLE at a concentration of 3% showed the highest inhibition percentage of fungal growth (IPFG) of B. oryzae, followed by S. terebinthifolius EO at 3% and 2%, with IPFG values of 80%, 74.44%, and 71.66%, respectively. At a concentration of 3%, branch oils from S. terebinthifolius and P. halepensis were found to have the highest IPFG values with 45.55% and 40.55%, respectively, against F. oxysporum growth. Moderate to weak activity was found against F. solani when S. terebinthifolius EO and P. halepensis OLE were applied to wood. EO and OLE-treated wood samples at 3% produced inhibitions of 54.44% and 41.11%, respectively, against R. solani.