Abstract
THE investigation of four extracts of Siwi date fruit (70% methanol, ethyl acetate, n-butanol and chloroform) showed good antibacterial, antifungal activities. This study includes evaluation of the antioxidant, and antimicrobial activities with determination of MIC for the four prepared extracts. Moreover, their cytotoxicity was determined against HeLa and HEK293T cancer cell lines by MTT 3-(4, 5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) assay. The antioxidant activity was determined by diphenyl-(2,4,6-trinitrophenyl) iminoaxonium (DPPH) method. New findings are the important elements as Ni and Si beside the essential valuable amino acid valine, and the pentoses xylose and arabinose all proved to exist in Siwi fruit never reported in any other Egyptian date varieties. Other important elements and secondary metabolites are seven unsaturated fatty acids, one phenolic acid, five flavonoids, sixteen amino acids, twenty monoterpenes and five sesquiterpenes beside an appreciable amount of dietary fibers, were also detected. No aflatoxins or pesticides were found in Siwi date fruit. Separation of all compounds was done through extensive chromatographic analysis on PC, CC and HPLC, Identification of all compounds was performed by UV, Co-PC with standards, amino acid analyzer, atomic absorption, GCMS and ESIMS. HPLC was used for the test of aflatoxins with aflatoxins standard, according to association of analytical communities AOAC. Furthermore, the test for pesticides was done using LC/MS/MS according to the European Standard method EN 15662: 2008.